Welcome to the

Du Laboratory

Overview

Structural Biology and Electrophysiology

The laboratory of Dr. Juan Du is focused on understanding the mechanism and pharmacology of excitatory neuronal receptors, which are critically involved in numerous neurological diseases. A combined approach of single particle cryo-EM, patch clamp electrophysiology and X-ray crystallography are employed to investigate the atomic structures and biological functions of these ion channel receptors.

Visit the Du and Lü labs joint website here.

Lab Funding

NIH R01 NINDS
Pew Scholars in Biomedical Sciences
Sloan Research Fellowship
McKnight Scholar Award for Neuroscience
Klingenstein-Simons Fellowship Award
American Heart Association Postdoctoral Fellowship (Dr. Zheng Ruan)

 

 

Join our team

Two NIH-funded postdoctoral positions and one senior research scientist position (patch-clamp electrophysiology) are available in the laboratory of Dr. Juan Du at Van Andel Institute. The Du Laboratory is interested in understanding how human body senses external stimuli, conveys the signals to the brain and generates responses via neuronal ion channels. To address these fundamental questions, our lab uses a diverse set of approaches to study the structure and function of these important ion channels by combining single-particle cryo-EM, patch-clamp electrophysiology, X-ray crystallography, and other function approaches (for examples, see articles Huang Y et al 2019; Fan C et al 2018; Winkler PA et al 2017). Experience with electrophysiology and/or protein biochemistry is preferred. Interested persons should send a CV and a cover letter to Dr. Du.

Contact Dr. Du

Our Impact

We’re raising thousands to save millions.

We’re turning hope into action for the millions of people around the world affected by diseases like cancer and Parkinson’s. Find out how you can help us make a difference.

  • 171 studies published from Nov. 1, 2020 to Oct. 1, 2021
  • 68 studies in high-impact journals from Nov. 1, 2020-Oct. 1, 2021
  • 41 clinical trials launched

Juan Du, Ph.D.

Associate Professor, Department of Structural Biology

Areas of Expertise

Structural biology, cryo-EM, synaptic receptors, ion channels

Biography

Dr. Juan Du earned her Diploma degree in chemistry from University of Goettingen and a Ph.D. from the Institute of Organic Chemistry and Biochemistry at the University of Freiburg in Germany. While there, she determined the structure of a key enzyme required for gluconeogenesis and revealed new facets of the membrane transporter FocA. In 2012, she joined the Gouaux Lab at Oregon Health & Science University’s Vollum Institute, where her work focused on characterizing excitatory triheteromeric NMDA receptor as well as inhibitory glycine receptor using cryo-EM and electrophysiology. She is now an assistant professor in the Center for Cancer and Cell Biology at Van Andel Institute.

Honors and Awards

  • 2020 Pew Scholars in Biomedical Sciences
  • 2020 Sloan Research Fellow in Neuroscience
  • 2019 Klingenstein-Simons Award in the Neuroscience
  • 2019 McKnight Scholar Award for neuroscience
  • 2018 Van Andel Institute Employee Impact Fund
  • 2012 Arthur Lüttringhaus Prize
  • 2011 Chinese Government Award for Outstanding Self-Financed Students Abroad

SELECTED PUBLICATIONS

*co-first author
# co-corresponding author

Ruan Z*, Haley E*, Orozco I*, Sabat M, Myers R, Roth R, Du J#, Lü W#. 2021. Structures of the TRPM5 channel elucidate mechanisms of activation and inhibitionNat Struct Mol Biol.

Yu J*, Zhu HT*, Lape R, Greiner T, Du J, Lü W, Sivilotti L#, Gouaux E#. 2021. Mechanism of gating and partial agonist action in the glycine receptorCell.

Ruan Z*, Osei-Owusu J*, Du J, Qiu Z#, Lü W#. 2020. Structures and pH-sensing mechanism of the proton-activated chloride channelNature.

Ruan Z, Orozco IJ, Du J#, Lü W#. 2020. Structures of human Pannexin 1 reveal ion pathways and mechanism of gating. Nature.

Huang Y, Fliegert R, Guse AH, Lü W#, Du J#. 2019. A structural overview of the ion channels of the TRPM familyCell Calcium.

Choi W*, Clemente N*, Sun W, Du J#, Lü W#. 2019. The structures and gating mechanism of human calcium homeostasis modulator 2Nature.

Haung Y, Roth B, Lü W#Du J#. 2019. Ligand recognition and gating mechanism through three ligand-binding sites of human TRPM2 channeleLife.

Haley E, Choi W, Fan C, Sun W, Du J, Lü W. 2019. Expression and purification of the human lipid-sensitive cation channel TRPC3 for structural determination by single-particle cryo-electron microscopyJ Vis Exp (143):e58754, doi:10.3791/58754.

Huang Y, Winkler PA, Sun W, Lü W#Du J#. 2018. Architecture of the TRPM2 channel and its activation mechanism by ADP-ribose and calciumNature.

Fan C*, Choi W*, Sun W, Du J#, Lü W#. 2018. Structure of the human lipid-gated cation channel TRPC3eLife:e36852.

Winkler PA*, Huang Y*, Sun W*, Du J, Lü W. 2017. Electron cryo-microscopy structure of a human TRPM4 channelNature.

Lü W*, Du J*, Goehring A, Gouaux E. 2017. Cryo-EM structures of the triheteromeric NMDA receptor and its allosteric modulationScience 355.

Du J*, Lü W*,Wu S, Cheng Y, Gouaux E. 2015. Glycine receptor mechanism elucidated by electron cryo-microscopyNature 526(7572):224–229.

Lee CH, Lü W, Michel JC, Goehring A, Du J, Song X, Gouaux E. 2014. Structures of NMDA receptor reveal subunit arrangement and pore architectureNature 511(7508):191–197.

Lü W, Du J, Schwarzer N, Wacker T, Andrade SLA, Einsle O. 2013. The formate/nitrite transporter family of anion channelsBiol Chem 394(6):715–727.

Lü W, Schwarzer N, Du J, Gerbig-Smentek E, Andrade SLA, Einsle O. 2012. Structural and functional characterization of the nitrite channel NirC from Salmonella typhimuriumProc Natl Acad Sci U S A 109(45):18395–18400.

Lü W, Du J, Schwarzer N, Gerbig-Smentek E, Einsle O, Andrade SLA. 2012. The FNT family channel FocA exports the products of mixed-acid fermentationProc Natl Acad Sci U S A 109(33):13254–13259.

Du J*, Say R*, Lu Ẅ, Fuchs G, Einsle O. 2011. Active site remodeling in the bifunctional fructose–1,6–bisphosphate aldolase/phosphataseNature 478(7370):534–537.

Lü W, Du J, Wacker T, Gerbig-Smentek E, Andrade SLA, Einsle O. 2011. Mechanism of gating in the formate channel FocA from Salmonella typhimuriumScience 332:352–354.

Lü W, Du J, Stahl M, Tzivelekidis T, Belyi Y, Gerhardt S, Aktories K, Einsle O. 2010. Structural basis of the action of glucosyltransferase Lgt1 from Legionella pneumophilaJ Mol Biol 396(2):321–331.

Emery Haley

Van Andel Institute Graduate School Student

Thesis: Structural, biochemical and pharmacological analysis of TRP channels in chemosensation and signaling

Emma Krueger

Assistant Research Technician

Sushant Kumar, Ph.D.

Postdoctoral Fellow

Ian Orozco, Ph.D.

Senior Research Scientist

Becca Roth

Assistant Research Technician

Zheng Ruan, Ph.D.

Postdoctoral Fellow

Ion channels, single-particle cryo-electron microscopy (cryo-EM), electrophysiology

Shannon Stewart

Assistant Research Technician

Xinyu Ye

Assistant Research Technician